Professor Sangeeta Tanna

Job: Professor of Pharmaceutical Analysis

Faculty: Health and Life Sciences

School/department: Leicester School of Pharmacy

Address: De Montfort, University, The Gateway, Leicester, LE1 9BH.

T: +44 (0)116 207 8274

E: stanna@dmu.ac.uk

W: www.dmu.ac.uk/research/research-faculties-and-institutes/health-and-life-sciences/dried-blood-spot-analysis/dried-blood-spot-analysis.aspx

Social Media:

 

Personal profile

Prof. Sangeeta Tanna's main interests and expertise are in the following areas:

  • Counterfeit drugs/medicines
  • Quality assurance of medicines
  • Dried blood spot (DBS) analysis
  • Quantitative bioanalysis
  • Clinical mass spectrometry
  • LC-HRMS
  • LC-MS/MS
  • Medicine adherence
  • Therapeutic drug monitoring
  • Clinical pharmacy practice
  • Paediatric pharmacokinetics
  • Paediatric biomarkers
  • Detection of food adulterants

 

She is particularly interested in the application of analytical techniques to the determination of low levels of target analytes, principally involving improvements to healthcare of patients. There include:

  • Dried blood spot (DBS) analysis for paediatric pharmacokinetic studies and patient care
  • Assessment of adherence to cardiovascular medications using DBS analysis
  • Investigation, detection and analysis of counterfeit medicines
  • Development of in vitro characterisation and analytical methods for a novel glucose-responsive, self-regulated insulin delivery system.

 

Prof. Tanna is the Athena SWAN Lead for the Leicester School of Pharmacy and Chair of the Pharmacy Athena SWAN self-assessment team (SAT).

 

Research group affiliations

Pharmacy Practice Research Group

Publications and outputs 

  • Quantitative screening of the pharmaceutical ingredient for the rapid identification of substandard and falsified medicines using reflectance infrared spectroscopy
    Quantitative screening of the pharmaceutical ingredient for the rapid identification of substandard and falsified medicines using reflectance infrared spectroscopy Lawson, Graham; Ogwu, John; Tanna, Sangeeta The World Health Organization suggests that approximately 10% of medicines worldwide are either falsified or substandard with higher figures in low and middle income countries. Such poor quality medicines can seriously harm patients and pose a threat to the economy worldwide. This study investigates attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectroscopy as a simple and rapid method for determination of drug content in tablet dosage forms. Paracetamol was used as the model pharmaceutical ingredient. Spectra of standard mixtures of paracetamol with different excipients formed the basis for multivariate PLS based quantitative analysis of simulated tablet content using different selected infrared absorbance bands. Calibration methods using ATR-FTIR were compared with the ATR-FTIR and conventional ultraviolet spectroscopic analyses of real tablet samples and showed that the paracetamol/microcrystalline cellulose mixtures gave optimum results for all spectral bands tested. The quantitative data for band 1524-1493cm-1 was linear (R2 ˃ 0.98; LOQ ≥ 10%w/w tablet). Global examples of paracetamol tablets were tested using this protocol and 12% of the tablet samples examined was identified as substandard. Each sample analysis was completed in just a few minutes. ATR-FTIR can therefore be used in the rapid screening of tablet formulations. The simplicity of the proposed method makes it appropriate for use in low and middle income countries where analytical facilities are not available. open access journal
  • Volumetric absorptive microsampling (VAMS) coupled with high-resolution, accurate-mass (HRAM) mass spectrometry as a simplified alternative to dried blood spot (DBS) analysis for therapeutic drug monitoring of cardiovascular drugs
    Volumetric absorptive microsampling (VAMS) coupled with high-resolution, accurate-mass (HRAM) mass spectrometry as a simplified alternative to dried blood spot (DBS) analysis for therapeutic drug monitoring of cardiovascular drugs Tanna, Sangeeta; Alalaqi, Ahmed; Bernieh, Dennis; Lawson, Graham Here, volumetric absorptive microsampling (VAMS), used for the measurement of cardiovascular drugs, is compared against conventional dried blood spot (DBS) card sampling to evaluate adherence to prescribed medication. Volumetric absorptive microsampling (VAMS) is an attractive alternative to plasma sampling for routine drug monitoring and potentially overcomes haematocrit issues associated with quantitative bioanalysis of conventional dried blood spots. A quantitative VAMS-based LC-HRAM MS assay for atenolol, lisinopril, simvastatin and valsartan was developed and validated. The assay demonstrated acceptable linearity, selectivity, accuracy, precision, recovery and insignificant matrix effects with no impact of haematocrit on assay accuracy. Volunteers provided both VAMS and DBS 903 card samples (the current standard) to allow comparison of the two methods and demonstrate the potential utility of VAMS. Analysis of VAMS samples correctly identified drugs in volunteers known to be adherent, and found no false positives from volunteers known to be taking no medication. There was a strong correlation between the two sampling systems confirming the utility of VAMS. Therapeutic drug monitoring (TDM) can assist clinicians in deciding how to proceed with treatment in the event of poor improvement in patient health. VAMS could offer a potentially more efficient method of sample collection, with fewer rejected samples than the DBS approach. The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.
  • Blood spot micro-samples analysed by LC-HRMS to monitor cardiovascular drug levels in patient samples from Iraq.
    Blood spot micro-samples analysed by LC-HRMS to monitor cardiovascular drug levels in patient samples from Iraq. Tanna, Sangeeta; Alalaqi, Ahmed; Bernieh, Dennis; Obaid, Yaseen; Lawson, Graham
  • Cardiovascular drug monitoring using quantitative LC-HRMS analysis of self-collected micro-volume blood samples
    Cardiovascular drug monitoring using quantitative LC-HRMS analysis of self-collected micro-volume blood samples Tanna, Sangeeta; Bernieh, Dennis; Alalaqi, Ahmed; Lawson, Graham Background Evidence suggests that ˃50% of cardiovascular (CVD) disease patients do not adhere to treatment thus impacting on patient health, additional healthcare costs and medicines wastage. DBS microsampling combined with LC-ToF MS detection has the potential to offer a simple means to monitor drug levels to enable clinicians to personalise optimum treatment for patients. This research compared the use of Whatman 903 dried blood spot (DBS) sample collection cards with volumetric absorptive micro-sampling (VAMS)/Mitra® technology for use as a personal sampling methodology. Methods Recruited volunteers were given demonstrations and information sheets concerning the investigation and sample collection. A liquid chromatography-high resolution mass spectrometry (LC-HRMS) method was validated for the determination of the top 11 UK prescribed cardiovascular drugs. For the preparation of DBS and VAMS calibration samples whole blood was spiked with different levels of the 11 target analytes. 8mm DBS discs or the absorptive VAMS tips were extracted with methanol containing the internal standard. The bioanalytical method was applied to fingerprick samples taken from volunteers some of whom were prescribed one or more of the target drugs. Volunteers not prescribed drugs represented blank samples. Results Approximately 17% of the DBS spots were unacceptable for quantification whereas 1 VAMS sample tip was rejected due to incomplete collection. Validation showed comparable quantitative results between the DBS and VAMS microsampling methods for the 11 target drugs. For two study groups anticipated cardiovascular drugs were detected in 83% of the pre-warned group and 73% of the trial group. The latter figure was higher than expected possibly due to the ‘white coat compliance’. The detected drug levels were in line with literature values for the half-life and Cmax for a given drug, Non-adherence was not uniform amongst the cardiovascular drugs. All volunteers preferred the VAMS methodology. Conclusions Both microsampling methods coupled with LC-HRMS analyses facilitate the identification of patients where the prescription apparently failed to produce detectable drug levels in the blood. This information should inform clinicians how to proceed in the healthcare process in the event of poor patient progress.
  • Comparison of VAMS and card based microsampling with LC-HRMS analysis to assess cardiovascular drug levels
    Comparison of VAMS and card based microsampling with LC-HRMS analysis to assess cardiovascular drug levels Bernieh, Dennis; Lawson, Graham; Tanna, Sangeeta Summary: An objective means of assessing medication adherence is needed to enable patients get the maximum therapeutic benefits from their medication. A bioanalytical assay using dried blood spot (DBS) and volumetric absorptive microsampling (VAMs) followed by liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses was developed and validated for quantification of 11 commonly UK prescribed cardiovascular drugs. Results from the two sampling devices showed that VAMs overcomes the limitations associated with using DBS cards for quantitative analyses. Introduction: Evidence suggests that ˃50% of heart disease patients do not adhere to treatment. This means half of the 370 million prescriptions dispensed for cardiovascular diseases (CVD) in the UK yearly, are wasted [1]. Wasted (unused) medicines cost the NHS up to £4 billion annually. Treatment success is also affected by variations in individual drug metabolism, drug-drug interactions and selecting appropriate dosage. Dried matrix microsampling combined with LC-HRMS detection has the potential to offer an objective means of assessing medication adherence [1, 2] to enable clinicians to optimise and personalise treatment for patients. However challenges in using DBS hampers the attractiveness of this technique. Novel microsamplers have been developed to overcome the limitations with DBS cards when a sub punch is used. This work compares the performance of the novel VAMs device and the traditional DBS card in terms of quantification for 11 cardiovascular drugs in a medication adherence study. Method: Chromatographic analyses were performed on a Zorbax column using gradient elution with a run time of 2.5 min. Identification was carried out using electrospray ionisation (positive) on an Agilent 6530A QTOF mass spectrometer. For the preparation of DBS calibration samples whole blood was spiked with the 11 target analytes to produce 30µl blood spots on specimen cards and dried. 8mm discs were punched out from DBS cards and the whole substrate was used for VAMs. Spots were extracted with methanol containing the internal standard. The developed and validated bioanalytical method was applied to finger prick blood samples taken from adult patients previously administered one or more of the target drugs. Results: The method validation showed good linearity. The accuracy (relative error) and precision (coefficient of variation) values were within the pre-defined limits of ≤15% at all tested concentrations for the 11 target drugs on both sampling devices. Haematocrit effect were significant on DBS card, but not on the VAMs device. Drug recoveries from spiked blood spots were ≥62% for amlodipine and simvastatin and ≥82% for the other target drugs. The assay sensitivity was sufficient to detect the drop in blood concentration, for all target drugs, resulting from missed doses. Conclusion: Ease of sampling data collected from participants’ show that VAMs devices was more user friendly compared to the DBS card. The developed microsampling based LC-HRMS assay has the potential to both assess medication adherence and to allow re-interrogation of the HRMS data collected for heart disease patients. This will enable health practitioners to monitor medication adherence. The method has potential to save the NHS money in wasted medicines and will allow health practitioners to personalize and optimize drug treatments for their patients.
  • Counterfeit or just poor quality?
    Counterfeit or just poor quality? Armitage, Rachel; Lawson, Graham; Tanna, Sangeeta Counterfeit medicines are a major public health issue. The World Health Organisation suggests that the occurrence of counterfeits range from 1% in high income countries to as much as ~30-40% in low to middle income countries, however many of these may be substandard rather than counterfeit due to poor quality control. This research investigates the potential of Attenuated Total Reflectance Fourier Transform Infrared (ATR FTIR) techniques to provide rapid quantitative analysis of suspect tablet formulations. Sample preparation for ATR FTIR is minimal and only requires a single tablet to be ground to a fine powder prior to analysis. This enables more rapid analysis of tablets. Reference spectra of the active pharmaceutical ingredient (API) and excipients were recorded for identification purposes. Using atenolol as a model, quantitative data was obtained from calibrated mixtures of API and excipients. Tablets from various countries, India, Saudi Arabia, Nepal and UK were analysed using both ATR FTIR and reference UV analyses. Results demonstrated that the API was detectable down to ca 5% w/w of the tablet. Several examples of poor dosage quality control were identified.
  • Performance of two different microsamplers for the LC-HRMS analysis of 10 cardiovascular drugs
    Performance of two different microsamplers for the LC-HRMS analysis of 10 cardiovascular drugs Bernieh, Dennis; Lawson, Graham; Tanna, Sangeeta Introduction: Evidence suggests that ˃50% of heart disease patients do not adhere to treatment. This means half of the 370 million prescriptions dispensed for cardiovascular diseases (CVD) in the UK yearly, are wasted [1]. Wasted (unused) medicines cost the NHS up to £4 billion annually. Treatment success is also affected by variations in individual drug metabolism, drug-drug interactions and selecting appropriate dosage. Dried matrix microsampling combined with LC-HRMS detection has the potential to offer an objective means of assessing medication adherence [1, 2] to enable clinicians to optimise and personalise treatment for patients. However challenges in using DBS hampers the attractiveness of this technique. Novel microsamplers have been developed to overcome the limitations with DBS cards when a sub punch is used. This work compares the performance of the novel volumetric absorptive microsampling (VAMS) device and the traditional DBS card in terms of quantification for 11 cardiovascular drugs in a medication adherence study. Method: Chromatographic analyses were performed on a Zorbax column using gradient elution with a run time of 2.5 min. Identification was carried out using electrospray ionisation (positive) on an Agilent 6530A QTOF mass spectrometer. For the preparation of DBS calibration samples whole blood was spiked with the 11 target analytes to produce 30µl blood spots on specimen cards and dried. 8mm discs were punched out from DBS cards and the whole substrate was used for VAMs. Spots were extracted with methanol containing the internal standard. The developed and validated bioanalytical method was applied to finger prick blood samples taken from adult patients previously administered one or more of the target drugs. Results: The method validation showed good linearity. The accuracy (relative error) and precision (coefficient of variation) values were within the pre-defined limits of ≤15% at all tested concentrations for the 11 target drugs on both sampling devices. Haematocrit effect were significant on DBS card, but not on the VAMs device. Drug recoveries from spiked blood spots were ≥62% for amlodipine and simvastatin and ≥82% for the other target drugs. The assay sensitivity was sufficient to detect the drop in blood concentration, for all target drugs, resulting from missed doses. Conclusion: Ease of sampling data collected from participants’ show that VAMs devices was more user friendly compared to the DBS card. The developed microsampling based LC-HRMS assay has the potential to both assess medication adherence and to allow re-interrogation of the HRMS data collected for heart disease patients. This will enable health practitioners to monitor medication adherence. The method has potential to save the NHS money in wasted medicines and will allow health practitioners to personalize and optimize drug treatments for their patients.
  • Quantitative LC-HRMS determination of selected cardiovascular drugs, in dried blood spots, as an indicator of adherence to medication
    Quantitative LC-HRMS determination of selected cardiovascular drugs, in dried blood spots, as an indicator of adherence to medication Bernieh, Dennis; Lawson, Graham; Tanna, Sangeeta Dried blood spot (DBS) sampling was investigated as a means of obtaining micro-volume blood samples for the quantitative analyses of ten commonly UK prescribed cardiovascular drugs as an indicator of medication adherence. An 8 mm disc was punched out from each DBS from calibration, quality control and volunteer samples and extracted using methanol containing the internal standard. Each extract was evaporated to dryness, the residue reconstituted in methanol:water (40:60 v/v) containing 0.1% formic acid and analysed by LC-HRMS. Chromatography was performed using gradient elution on a Zorbax Eclipse C18 HD 100 mmx2.1 mm, 1.8 µm pore size column with the column oven temperature at 40˚C. Flow rate of the mobile phase was 0.6ml/min with a run time of 2.5 min. Electrospray positive ionization was used for MS detection. Drug recoveries from spiked blood spots were 68% for simvastatin and ≥ 87% for all other target drugs. Compound specificity was obtained operating the MS with a 5ppm mass window. The LC-HRMS method was validated, with results for accuracy and precision within acceptable limits; analytes were stable at room temperature for at least 10 weeks and different blood spot volumes and haematocrit values had no significant effect. The LC-HRMS assay was used to analyse DBS samples from volunteers, some of whom were prescribed one or more of the target drugs. In results from 37 volunteers the assay successfully identified volunteers who were known to be either adherent or nonadherent; confirmed the correct drug/drugs for multiple prescriptions; demonstrated no false positives from other cardiovascular drugs; revealed several examples of unsuspected non-adherence. These results indicated that the developed assay was suitable for trials with patients. The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.
  • Quantitative LC-HRMS analysis of dried blood spots to assess adherence to cardiovascular pharmacotherapy
    Quantitative LC-HRMS analysis of dried blood spots to assess adherence to cardiovascular pharmacotherapy Lawson, Graham; Bernieh, Dennis; Tanna, Sangeeta The analysis of dried blood spot samples (DBS) by liquid chromatography – high resolution mass spectrometry (LC-HRMS) for assessing adherence to candidate cardiovascular therapeutic drugs was investigated. To evaluate the method 8 mm discs were punched from each DBS and extracted followed by subjecting to LC-HRMS analysis. Trials using the top 11 UK prescribed cardiovascular drugs are reported demonstrating the ability of the system to detect the target analytes during the 24 hour repeat prescription cycle. The system responded successfully to challenges by samples from volunteers of known adherence or receiving no medication. Examples of non-adherence to different medications were identified.
  • LC-HRMS analysis of 133 patient micro-volume blood samples to allow clinical assessment of medication adherence
    LC-HRMS analysis of 133 patient micro-volume blood samples to allow clinical assessment of medication adherence Tanna, Sangeeta; Alalaqi, Ahmed; Bernieh, Dennis; Lawson, Graham 133 DBS card/VAMS micro-volume blood samples were analysed by LC-HRMS to determine if therapeutic levels of the prescribed cardiovascular (CVD) drugs were present. This research focussed on the top 11 UK prescribed CVD drugs. Calibration samples, prepared from spiked human whole blood, were extracted and analysed by LC-HRMS. This method was validated and applied to volunteer and patient samples. The system successfully identified the prescribed drugs in volunteer’s samples who were known to be adherent. Non-detection of a prescribed drug indicated non-adherence to prescription: a situation identified for 12% of the volunteers and 36% of the patients.

Click here for a full listing of Sangeeta Tanna's publications and outputs.

Areas of teaching

  • Pharmaceutics
  • Biopharmaceutics
  • Novel drug delivery systems
  • Paediatric formulations and drug delivery
  • Geriatric pharmacotherapy
  • Substandard/falsified medicines
  • Therapeutic drug monitoring
  • Assessment of medication adherence using analytical science
  • Bioanalysis for personalised drug dosing
  • How to design research posters

Courses taught

  • MPharm Pharmacy
  • BSc Pharmaceutical and Cosmetic Science
  • MSc Pharmaceutical Quality by Design
  • Postgraduate workshop training session on “How to design effective posters”.

Honours and awards

APS PharmSci Geoffrey Phillips Analytical Science Commendation Prize 2016
Counterfeit medicines: Rapid quantification of active pharmaceutical ingredients in tablet formulations.
Ogwu J, Lawson G, Tanna S
APS 7th International PharmSci Conference. Glasgow, UK. 5-7 September 2016 

Nominated for Times Higher Education (THE) Research Project of the Year 2012
Blood Spot Analysis.
Tanna S and Lawson G
November 2012

Royal Society of Chemistry – Analytical Methods Prize 2010
Analysis of dried blood spots – the potential for paediatric pharmacokinetic studies of captopril.
Tanna S, Pandya H, Mulla H, Titman C, and Lawson G.
RSC Analytical Research Forum. Loughborough University. 26-28 July 2010 

British Pharmaceutical Conference 2009 – Science Poster Prize
The use of blood spot analysis in paediatric care - From laboratory to bedside and back again.
Lawson G, Mulla H, Pandya H, Tanna S.
Science @ BPC2009 Royal Pharmaceutical Society. Manchester Central. Sept 7-9 2009. 

Runner-up for the 2003 Westminster Medal & Prize for best research poster
The design of a self-regulated insulin delivery system
Tanna S, Sahota T, Taylor MJ.
SET for Britain - The House of Commons Reception for Britain’s Top Younger Scientists, Engineers and Technologists, The House of Commons, Westminster. 17 March 2003

Membership of professional associations and societies

  • British Mass Spectrometry Society
  • Academy of Pharmaceutical Sciences
  • Royal Society of Chemistry

Conference attendance

Invited Lectures

Lawson G and Tanna S. Identifying medication nonadherence could save £billions. CPSA Europe 2019. Cambridge, UK. 5-8 February 2019.

Tanna S, Alalaqi A, Lawson G. Monitoring of cardiovascular drug levels by quantitative LC-HRMS analysis of patient collected micro-volume blood samples. World Conference on Analytical and Bioanalytical Chemistry. Barcelona, Spain. 23-24 July 2018.

Lawson G, Ogwu J, Armitage R, Tanna S. Counterfeit tablet investigations: are Raman and ATR FT/IR techniques for the real world? World Conference on Analytical and Bioanalytical Chemistry. Barcelona, Spain. 23-24 July 2018.

Tanna S. Assessment of medication nonadherence – A UK perspective. ‘Internationalization@Home’ Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium. 2nd May 2018.

Bernieh D, Lawson G, Tanna S. Development of an evidence based assessment of medication adherence for heart disease patients. APS 7th International PharmSci Conference. Glasgow, UK. 5-7 September 2016

Ogwu J, Lawson G, Tanna S. Counterfeit medicines: Rapid quantification of active pharmaceutical ingredients in tablet formulations. APS 7th International PharmSci Conference. Glasgow, UK. 5-7 September 2016

Bernieh D, Lawson G, Tanna S. Quantitative LC-ToF MS analyses of dried blood spots: Developing an assessment of medication adherence for heart disease patients. The Reid Bioanalytical Forum. Surrey, UK. September 2015

Tanna S and Lawson G. Adherence to medication assessed using dried blood spot analysis. Invited lecture at the 5th International Conference and Exhibition on Analytical & Bioanalytical Techniques. Beijing, China 18-20 August 2014

Lawson G and Tanna S. Counterfeit tablet investigations: Is ATR FT/IR A technique for the real world. Invited lecture at the 5th International Conference and Exhibition on Analytical & Bioanalytical Techniques. Beijing, China 18-20 August 2014 

Tanna S and Lawson G. Cardiovascular drug medication compliance assessed by dried blood spot sampling techniques.  Invited lecture at the 4th International Conference and Exhibition on Analytical Techniques. Las Vegas, USA 15-17 October 2013

Lawson G, Armitage R, Alhedethe A and Tanna S. Rapid identification of counterfeit pills by ATR FT/IR analysis of crushed samples. Invited lecture at the 4th International Conference and Exhibition on Analytical Techniques. Las Vegas, USA 15-17 

Tanna S and Lawson G.  Blood Spot Analysis – Paediatrics to Pensioners. Invited public lecture at the Festival of Ideas, De Montfort University, Leicester UK, 23 April 2013

Lawson G, Armitage R, Tanna S.  Identification of Counterfeit Drugs. Invited public lecture at the Cafe Scientifique event, Nanjing, China, November 2012 

Tanna S and Lawson G.  Dried Blood Spot (DBS) Analysis for Healthcare Applications. Invited public lecture at the Cafe Scientific event, Nanjing, China, November 2012

Lawson G, Armitage R, Tanna S. Identification of counterfeit medicines. 7th Annual Pharmaceutical Anti-Counterfeiting Meeting. 2012, Visiongain Conference Centre, Barbican, London, UK. 26-27 June 2012.

Lawson G, Armitage R, Tanna S.  Identification of counterfeit medicines. Conference on International Biology and Medicine Innovative Industrialization (IBMII). Chongqing Convention Centre, Chongqing, China, April 2012.

Tanna S. and Lawson G. Dried blood spot (DBS) analysis for healthcare applicationsConference of International Biology and Medicine Innovative Industrialization (IBMII). Chongqing Convention Centre, Chongqing, China, April 2012

Lawson G, Ross G, Sage A, Mulla H, Pandya H and Tanna S.  Potential of the QToF in Paediatric Biomarking. Invited Lecture. 1st International Pediatric Biomarker Symposium. Congress Park, Igls Austria. 4-6 Feb 2010

Invited Poster Presentations

Tanna S, Patel P, Mulla H and Lawson G. Dried blood spot analysis – a comparison of SIM, MSMS and accurate mass TOF analyses for selected drugs. 27th LC-MS Montreux Symposium, Montreux, Switzerland, 10-12 November 2010

Tanna S, Pandya H, Mulla H, Titman C, and Lawson G. Analysis of dried blood spots – the potential for paediatric pharmacokinetic studies of captopril. RSC Analytical Research Forum. Loughborough University. 26-28 July 2010

Lawson G, Mulla H, Ross G, Sage A and Tanna S. Accurate mass versus tandem mass measurement of paediatric biomarkers. RSC Analytical Research Forum. Loughborough University. 26-28 July 2010

Tanna S, Pandya H, Mulla H, Titman C, and Lawson G.  Analysis of dried blood spots – the potential for paediatric pharmacokinetic studies of captopril. 1st International Pediatric Biomarker Symposium. Congresspark. Igls, Austria. 4-6 Feb 2010.

Poster Presentations

Alalaqi A, Lawson G, Obaid Y, Tanna S. Assessment on non-adherence to cardiovascular medications in Iraq by 8-items Morisky questionnaire and dried blood spot samples analysis. APS@FIP Conference, Glasgow, UK. 7th September 2018.

Tanna S, Alalaqi A, Bernieh D, Obaid Y, Lawson G. Blood spot micro-samples analysed by LC-HRMS to monitor cardiovascular drug levels in patient samples from Iraq. 10th Medical Conference of Misan Health Directorate, Misan, Iraq. 5-7 December 2017.

Tanna S, Bernieh D, Alalaqi A, Lawson G. Cardiovascular drug monitoring using quantitative LC-HRMS analysis of self-collected micro-volume blood samples. 15th International Congress of Therapeutic Drug Monitoring & Clinical Toxicology 2017, Kyoto, Japan. 24-27 September 2017.

Bernieh D, Lawson G, Tanna S. Comparison of VAMS and card based microsampling with LC-HRMS analysis to assess cardiovascular drug levels. Mass Spectrometry: Applications to the Clinical Lab (MSACL) EU 2017. Salzburg, Austria. 10-14 September 2017.

Armitage R, Lawson G, Tanna S. Counterfeit or just poor quality control? APS 8th International PharmSci Conference. Hatfield, UK. 5-8 September 2017.

Bernieh D, Lawson G, Tanna S. Performance of two different microsamplers for the LC-HRMS analysis of 11 cardiovascular drugs. The Reid Bioanalytical Forum. Cambridge, UK. 5-7 September 2017.

Tanna S, Alalaqi A, Bernieh D, Lawson G. LC-HRMS analysis of 133 patient micro-volume blood samples to allow clinical assessment of medication adherence. Mass Spectrometry: Applications to the Clinical Lab (MSACL) 2017. Palm Springs, USA. 22-26 January 2017.

Lawson G, Bernieh D, Tanna S. Quantitative LC-HRMS analysis of dried blood spots to assess adherence to cardiovascular pharmacotherapy. Mass Spectrometry: Applications to the Clinical Lab (MSACL) 2017. Palm Springs, USA. 22-26 January 2017.

Tanna S, Bernieh D, Lawson G. Adherence to cardiovascular pharmacotherapy assessed by quantitative LC-HRMS analysis of dried blood spots. Advances in Clinical Analysis 2016, London, UK. 30 November 2016.

Tanna S, Bernieh D, Lawson G. Adherence to cardiovascular pharmacotherapy assessed by quantitative LC-HRMS analysis of dried blood spots. 21st International Mass Spectrometry Conference. Toronto, Canada. 20-26 August 2016.

Lawson G, Ogwu J, Armitage R, Alcroft C, Tanna S. Fast identification of counterfeit medicines – a comparison of two MS methods. 21st International Mass Spectrometry Conference. Toronto, Canada. 20-26 August 2016.

Tanna S, Bernieh D, Lawson G. Assessment of adherence to cardiovascular pharmacotherapy using quantitative dried blood spot analysis. Mass Spectrometry: Applications to the Clinical Lab (MSACL) 2015. Salzburg, Austria. 8-11 September 2015.

Lawson G and Tanna S. Liquid chromatography-high resolution mass spectrometry used for the analysis of dried blood spot samples in therapeutic drug monitoring. Mass Spectrometry: Applications to the Clinical Lab (MSACL) 2015. Salzburg, Austria. 8-11 September 2015.

Bernieh D, Lawson G, Tanna S. Development of an evidence based assessment of medication adherence for heart disease patients. APS 7th International PharmSci Conference. Glasgow, UK. 5-7 September 2016.

Ogwu J, Lawson G, Tanna S. Counterfeit medicines: Rapid quantification of active pharmaceutical ingredients in tablet formulations. APS 7th International PharmSci Conference. Glasgow, UK. 5-7 September 2016.

Bernieh D, Lawson G, Tanna S. It’s in a drop of blood – or is it? SET for BRITAIN Poster Presentations by Britain’s Top Younger Scientists, Engineers and Technologists at the House of Commons, Westminster, London, UK. 7 March 2016.

Tanna S, Bernieh D, Lawson G.  Assessment of adherence to cardiovascular pharmacotherapy using quantitative dried blood spot analysis. Mass Spectrometry: Applications to the Clinical Lab (MSACL) 2015. Salzburg, Austria. 8-11 September 2015.

Lawson G and Tanna S. Liquid chromatography-high resolution mass spectrometry used for the analysis of dried blood spot samples in therapeutic drug monitoring. Mass Spectrometry: Applications to the Clinical Lab (MSACL) EU 2015. Salzburg, Austria. 8-11 September 2015.

Bernieh D, Lawson G, Tanna S. Quantitative LC-ToF MS analyses of dried blood spots: Developing an assessment of medication adherence for heart disease patients. The Reid Bioanalytical Forum. Surrey, UK. September 2015.

Bernieh D, Lawson G, Tanna S. Quantitative dried blood spot analyses: An aid to medicine optimization for heart disease patients? The 6th International Conference and Exhibition on Analytical & Bioanalytical Techniques. Valencia, Spain. August 2015.

Ogwu J, Lawson G, Armitage R, Tanna S. Rapid instrumental detection and quantification of counterfeit pharmaceutical tablet formulations: is ATR-FTIR an option? The 6th International Conference and Exhibition on Analytical & Bioanalytical Techniques. Valencia, Spain. August 2015.

Bernieh D, Lawson G, Tanna S.Can quantitative dried blood spot analyses be an aid to medicine optimization in cardiovascular diseases? The Analytical Research Forum 2015. London, UK. 3rd July 2015.

Ogwu J, Lawson G, Armitage R, Tanna S.  ATR-FTIR for rapid detection and quantification of counterfeit medicines. The Analytical Research Forum 2015. London, UK.  3rd July 2015.

Ogwu J, Lawson G, Tanna S.  Rapid instrumental detection of counterfeit medicines – how feasible is it? The Forensic Institute Research Network (FIRN) conference. Derby, UK. April 24 2015.

Lawson G, Alcroft C, Baker A, Tanna S. Analysis of unknown powders and tablets using an atmospheric solids analysis probe and a compact mass spectrometer. The Forensic Institute Research Network (FIRN) conference. Derby, UK. April 24 2015.

Tanna S and Lawson G. Adherence to cardiovascular medications assessed using quantitative dried blood spot analysis. Proceedings of the 25th International Symposium on Pharmaceutical and Biomedical Analysis and the 10th International Symposium on Drug Analysis. Liege, Belgium. June 22-25 2014.

Lawson G, Turay E, Armitage R, Tanna S. Rapid identification of counterfeit medicines by ATR FT/IR. Proceedings of the 25th International Symposium on Pharmaceutical and Biomedical Analysis and the 10th International Symposium on Drug Analysis. Liege, Belgium. June 22-25 2014.

Tanna S, Armitage R, Lawson G. Identification of counterfeit pills - Is rapid instrumental analysis possible? Proceedings of The 24th International Symposium on Pharmaceutical and Biomedical Analysis. Bologna, Italy. 30 June-3 July 2013.

Lawson G, Cocks E, Tanna S. Liquid chromatography-high resolution mass spectrometry applied to therapeutic drug monitoring using DBS sampling. Proceedings of The 24th International Symposium on Pharmaceutical and Biomedical Analysis. Bologna, Italy. 30 June-3 July 2013.

Tanna S, Cocks E, Lawson G. Is the patient taking their 'heart' pills? A dried blood spot sample - LC-HRMS assay. Proceedings of The 24th International Symposium on Pharmaceutical and Biomedical Analysis. Bologna, Italy. 30 June-3 July 2013.

Tanna S, Patel P, Mulla H, Lawson G. Dried blood spot analysis to improve paediatric medication. Separation Science Asia. Singapore. 27-28 July 2011.

Lawson G, Cocks E. Tanna S.  High resolution mass spectrometry for quantitative analysis of dried blood spots. Separation Science Asia. Singapore, 27-28 July 2011.

Patel P, Pandya H, Spooner N, Della Pasque O, Gade S, Kairamknoda V, Lawson G, Tanna S, Mulla H. Dried blood spots and sparse sampling: A perfect combination for minimally invasive PK/PD studies in children. Population Approach Group Europe (PAGE) Meeting, Athens, Greece, June 2011.

Tanna S, Cocks E, Lawson G.  High resolution mass spectrometry for analysis of selected drugs in dried blood spots. 59th Conference on Mass Spectrometry and Allied Topics ASMS Meeting, Denver, Colorado, USA 5 – 9 June 2011.

Lawson G, Mulla H, Patel P, Tanna S. Examples of dried blood spot sampling and analysis to improve paediatric medicine. 59th Conference on Mass Spectrometry and Allied Topics. ASMS Meeting, Denver, Colorado, USA 5-9 June 2011.

Patel P, Lawson G, Tanna S, Mulla H. Facilitating paediatric PK studies: utility of the dried blood spot. Pharmacokinetics UK Conference, Birmingham UK, November 2009. 

Patel P, Lawson G, Mulla H, Tanna S. Applying dried blood spot analysis: the pathway to better paediatric care. Science @ BPC2009 Royal Pharmaceutical Society. Manchester Central, UK September 7-9 2009.

Lawson G, Pandya H, Mulla H, Tanna S. The use of blood spot analysis in paediatric care - From laboratory to bedside and back again. Science @ BPC2009 Royal Pharmaceutical Society. Manchester Central, UK. September 7-9 2009.

Lawson G, Coffey J, Titman C, Tanna S.  Dried blood spot analyses by Ion Trap – Time of Flight Mass Spectrometry, the potential for improved child care? 18th International Mass Spectrometry Conference, PWA 427, Bremen, Germany Aug 30 – Sept 4 2009.

Lawson G, Patel, P, Tanna S.  The use of dried blood spot analysis in paediatric care – how mass spectrometry can direct child medication. 18th International Mass Spectrometry Conference, PMM 468, Bremen, Germany Aug 30 – Sept 4 2009.

Tanna S, Lawson G Mulla H, Pandya H From laboratory to bedside and back again – The use of blood spot analysis in paediatric care. ACCP/ESCP International Congress on Clinical Pharmacy. Orange County Convention Centre, Orlando, Fl. USA. April 24 -28th.2009.

Tanna S, Sahota, TS, Sawicka K, Taylor MJ. UV polymerised dextran-concanavalin A acrylic derivatised gels for closed-loop insulin delivery. The 33rd International Annual Meeting & Exposition of the Controlled Release Society, Vienna, Austria, July 2006.

Sawicka K, Sahota TS, Taylor MJ, Tanna S. Development and application of a reversed-phase HPLC method for the analysis of components from a closed-loop insulin delivery system. The 33rd International Annual Meeting & Exposition of the Controlled Release Society, Vienna, Austria, July 2006.

Tanna S, Taylor MJ, Sahota TS. Novel glucose-sensitive gels for self-regulated insulin delivery. SET for BRITAIN Poster Presentations by Britain’s Top Younger Scientists, Engineers and Technologists at the House of Commons, Westminster, London, March 2003.

Tanna S, Sahota T, Clark J, Taylor MJ.   Rheological characterisation and insulin delivery of a novel glucose-sensitive gel with a carbomer carrier. Proceed. Intern. Symp. Control. Rel. Bioact. Mater. 28: 327-328. The 28th International Symposium on Controlled Release of Bioactive Materials, San Diego, California, USA, June 2001.

Clark J, Taylor MJ, Sahota TS, Tanna S.  Development of an “in-vivo” model for demonstrating the action of a responsive gel membrane to changes in blood glucose concentrations. Proceed. Intern. Symp. Control. Rel. Bioact. Mater. 28: 307-308. The 28th International Symposium on Controlled Release of Bioactive Materials, San Diego, California, USA, June 2001.

Tanna S and Taylor MJ.  Insulin delivery governed by lectin-glycogen gels sensitive to glucose. Proceed. Intern. Symp. Control. Rel. Bioact. Mater. 1998; 25: 737.

Tanna S and Taylor MJ.  Glucose-responsive gels based on dextran covalently coupled with lectin to control insulin delivery. J Pharm. Pharmac., 1997; 49 Suppl. 4: 76.

Taylor MJ Tanna S, Adams G. Insulin delivery using a novel glucose sensitive formulation. Proceed. Intern. Symp. Control. Rel. Bioact. Mater. 1995; 22.

Taylor MJ, Tanna S, Cockshot S,  Vaitha R. A novel self-regulated delivery system using unmodified solutes in glucose sensitive gel membranes. Proceed. Third European Symp. Control. Drug Delivery, The Netherlands. 1994; (240-5).

Current research students

4 PhD completions.

Currently supervising 2 PhD students in the areas of dried blood spot analysis and detection of counterfeit medicines.

We welcome enquiries from prospective PhD students.

Externally funded research grants information

Comparison of VAMS and card based microsampling with LC-HRMS analysis to assess cardiovascular drug levels. MSACL EU – Student Travel Bursary. Sept 2017.

Performance of two different microsamplers for the LC-HRMS analysis of 11 cardiovascular drugs. The Chromatographic Society – Student Bursary to attend Reid Bioanalytical Forum. Sept 2017.

Assessment of adherence to cardiovascular medications in Iraq by Morisky Questionnaire and dried blood spot analysis. Misan Health Directorate, Iraq Ministry of Health. Oct 2015- Sept 2019. Contract value £57,900.

Rapid detection of counterfeit medicines. Oct 2013 – Sept 2017. Self-funded PhD studentship form Nigeria.

Rapid detection of counterfeit medicines and their significance to the Kingdom of Saudi Arabia. Jan 2013 – Jan 2014. Saudi Arabia Embassy. Contract value £18,300.

Dried blood spot analysis. April 2012. British Council support on UK-Chongqing Collaboration on Technology Transfer and Research.

Assessment of compliance for cardiovascular medications using dried blood spot analysis. 2011 – 2012. The Gunn and Carter Fellowship
Collaborators UHL and University of Leicester. Contract  £26,000.

Pharmacokinetic investigation of caffeine in neonatal children during clinical care. 2008-2011.  Joint funding from GlaxoSmithKline/ UHL NHS Trust/DMU.  Collaborators GSK, UHL and University of Leicester.  Contract value £75,000.

Development of a model for monitoring therapeutic levels of dexamethasone in neonates via microanalysis. 2008 – 2011 UHL NHS Trust consumables funding .  Contract value £21,000.

Assessing the Bioequivalence of Unlicensed Liquid Captopril formulations used in the treatment of Children with Heart Failure. 2008-2010.  NIHR Research for Patient Benefit Funding.  Joint research with UHL NHS Trust.   Contract value £51,774.

Novel methods for paediatric drug monitoring using microanalysis. 2007 – 2008 NIHR NEAT Feasibility funding.  Contract value £68,296

The development of a totally implantable closed-loop insulin delivery device for the management of diabetes. 2004 –2006. National Institute for Health Research – New and Emerging Applications of Technology (NEAT) Programme. Contract value £187,000

Internally funded research project information

Identifying non-adherence to cardiovascular medications in Kenya. DMU GCRF Funded Project. March 2019-July 2019. £25,000

Helping people in Africa stay clear of fake medicines. #DMUengage #DMUglobal award. Nov 2016-July 2017. £2000.

Tandem Mass Spectrometry Project. Contract value £30,000. DMU HEIF Funded £30,000.

Tandem Mass spectrometry analyses of dried blood spots. 2010-13 Contract value £300,000. Role CI DMU RIF funded.

Development of a model for monitoring therapeutic levels of dexamethasone in neonates via microanalysis. DMU PhD Student Bursary CI and 2nd supervisor. Collaborators UHL NHS Trust, Sheffield Children’s Hospital, GSK plc. 2008-2011. Contract value £40,500.

Tandem Mass Spectrometry Project. Contract value £30,000. Role CI (with Dr G Lawson PI) DMU HEIF Funded £30,000.

Tandem Mass spectrometry analyses of dried blood spots. 2010-13 Contract value £300,000. Role CI (with Dr G Lawson PI) DMU RIF funded.

Development of a model for monitoring therapeutic levels of dexamethasone in neonates via microanalysis. DMU PhD Student Bursary CI and 2nd supervisor. Collaborators UHL NHS Trust, Sheffield Children’s Hospital, GSK plc. 2008-2011. Contract value £40,500.

Case studies

1.Blood spot analysis to assess adherence to cardiovascular pharmacotherapy in Iraq. This current research is in collaboration with hospitals is Iraq and the Misan Health Directorate. Patient DBS samples are analysed at DMU and the results are given to the collaborating clinicians in Iraq.

The impact of this research is threefold:

  • Improved patient health via optimised drug dosing regimens - a life and death situation
  • Clarity and personalised patient data to the clinician to provide an evidence-base for further treatment
  • Huge cost savings to the healthcare supplier by eliminating unnecessary future care and unnecessary hospital re-admissions

2. Under the auspices of #DMUengage and #DMU global Prof. Sangeeta Tanna and 3 undergraduate students from the Leicester School of Pharmacy travelled to Nairobi, Kenya on June 19-26 2017 to engage with various stakeholders about the research carried out by Prof. Tanna and Dr Graham Lawson on the rapid detection of substandard/falsified medicines. The trip included visits to:

  • Kenya National Quality Control Laboratory
  • Kenya Pharmacy and Poisons Board
  • Aga Khan University Hospital
  • Kenyatta National Hospital

The trip outcomes included:

  • The levels of counterfeits medicines confirmed to be greater than 40
  • Exchange of information on counterfeit/substandard medicines between DMU and  the above stakeholders in Kenya
  • There was interest in joint work to develop expertise in the identification of substandard medicines

3.The blood spot analysis research and its potential impact on improving healthcare received extensive media coverage during a British Council sponsored visit to Chongqing, China in April 2012. It was widely covered by 20 national and local media, including CCTV, Chongqing TV, Xinhua News Agency, Guang Ming Daily People’s Daily, HongKong Wenhui Po, people.com.cn, CQ.QQ.com etc. The link below is Chongqing TV’s coverage of the event at its satellite news programme.

http://v.youku.com/v_show/id_XMzg3Nzg2OTk2.html

4. In September 2011 the pioneering use of dried blood spot methods to monitor prescription compliance among patients taking cardiovascular medications was the subject of local and national media coverage. This included television coverage on BBC national and local news in addition to a live BBC Radio Leicester interview. It was featured on the BBC news website at: http://www.bbc.co.uk/news/uk-england-leicestershire-15002237

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