Dried Blood Spot Analysis
Dr Sangeeta Tanna and Dr Graham Lawson from the Pharmacy Practice Research Group have developed methods to identify and quantify therapeutic drugs present in a 5mm diameter dried blood spot collected on a sampling card. These micro-analyses of blood samples from a finger or heel prick use sophisticated liquid chromatography-high resolution mass spectrometry instrumentation.
In 2010, Drs Tanna and Lawson were awarded the Royal Society of Chemistry – Analytical Methods Prize for their research on the application of dried blood spot analysis to the improvement of paediatric medical care. These developed analytical methods have been applied to dried blood spot samples obtained from paediatric patients in University Hospitals of Leicester NHS Trust (UHL) and Sheffield Children’s Hospital and so the impact of this research on patients currently in care is already underway.
In 2012, their blood spot analysis research reached the final 6 in the prestigious Times Higher Education (THE) Award of Research Project of the Year.
An alarming fact is that, worldwide, at least 50% of all people needing medication do not take their medicine as prescribed. This is a global healthcare problem costing many patients their good health and adding unnecessary billions of pounds to healthcare system costs. Drs Tanna and Lawson have expanded their research to use the dried blood spot sampling technique to monitor adherence to prescribed drug therapy.
This work has come together in a book, written by Drs Tanna and Lawson and recently published by Elsevier entitled:
Analytical Chemistry for Assessing Medication Adherence
Tanna&Lawson book front cover
Dr Tanna and Dr Lawson’s work on the pioneering use of dried blood spot methods to monitor prescription adherence among patients taking cardiovascular medications has been published in a number of research articles and presented at international and national conferences. Cardiovascular disease (CVD) is one of the biggest killers worldwide affecting 1 in 3 adults in the UK and the current medical care of such patients is with a combination of cardiovascular drugs. There is evidence that up to 60% of patients ignore their medication or take it incorrectly. Initial results from a pilot study would seem to indicate a higher level of adherence but instances of taking medicines incorrectly have also been identified.
Accurate information on patient drug uptake will aid the clinical decision making process. Current results are based on dried blood spot samples collected in a laboratory and new research to validate a collection method for home use by the patient is currently underway. This research work is featured on a number of websites including: http://www.bbc.co.uk/news/uk-england-leicestershire-15002237
Bernieh D, Lawson G, Tanna S. Quantitative LC-HRMS determination of selected cardiovascular drugs, in dried blood spots, as an indicator of adherence to medication. Journal of Pharmaceutical and Biomedical Analysis 2017; 142:232-243. doi: 10.1016/j.jpba.2017.04.045.
Tanna S and Lawson G. In Thomas BF, ed. Analytical Chemistry for Assessing Medication Adherence. New York: Elsevier; 2016 ISBN: 978-0-12-805463-5.
Tanna Sand Lawson G. Self-sampling and quantitative analysis of dried blood spot samples – can it shift the balance in our over-burdened healthcare systems? Bioanalysis. 2015; 7(16):1963-1966.
Tanna, S., Bernieh, D. and Lawson G LC-HRMS analysis of dried blood spot samples for assessing adherence to cardiovascular medications. Journal of Bioanalysis and Biomedicine. 2015; 7(1):001-005. doi:10.4172/1948-593X.1000115.
Tanna S and Lawson G. Dried blood spot analysis to assess medication adherence and to inform personalization of treatment. Bioanalysis 2014; 6(21):2825-2838.
Tanna S and Lawson G. Cardiovascular drug medication adherence assessed by dried blood spot analysis. Journal of Analytical and Bioanalytical Techniques 2014; S12:006. doi: 10.4172/2155-9872.S12.006.
Lawson G, Cocks E, Tanna S. Bisoprolol, ramipril and simvastatin determination in dried blood spot samples using LC-HRMS for assessing medication adherence. Journal of Pharmaceutical and Biomedical Analysis 2013; 81-82:99-107.
Tanna S, Lawson G. Spot On. The Analytical Scientist 2013; April 2013: 22-27.
Lawson G, Patel P, Mulla H, Tanna S. Dried blood spot sampling with LC-MS analysis for routine therapeutic caffeine monitoring in neonates. ISRN Chromatography 2012; 2012: Article ID 828719, 7 pages, doi:10.5402/2012/828719.
Lawson G, Cocks E, Tanna S. Determination of atenolol in dried blood spot samples by LC-HRMS: A potential method for assessing medication adherence. Journal of Chromatography B 2012; 897:72-79.
Lawson G, Mulla H, Tanna S. Preliminary investigation of captopril in dried blood spots with liquid chromatography-mass spectrometry: A potential method for neonatal pharmacokinetic studies. Journal of Bioanalysis and Biomedicine 2012; 4(2):016-025.
Tanna S, Lawson G. Analytical methods used in conjunction with dried blood spots. Analytical Methods 2011; 3(8):1709-1718.
Patel P, Tanna S, Mulla H, Kairamkonda V, Pandya H, Lawson G. Dexamethasone quantification in dried blood spot samples using LC-MS: The potential for application to neonatal pharmacokinetic studies. Journal of Chromatography B 2010; 878:3277-3282.
Patel P, Mulla H, Tanna S, Pandya H. Facilitating pharmacokinetic studies in children: A new use of dried blood spots. Archives of Disease in Childhood 2010; 95(6):484-487.
Patel P, Lawson G, Mulla H and Tanna S. Applying dried blood spot analysis: the pathway to better paediatric care. Journal of Pharmacy and Pharmacology 2009;61(1 Suppl.): A112
Lawson G, Mulla H, Pandya H, Tanna S. The use of dried blood spot analysis in paediatric care – from laboratory to bedside and back again. Journal of Pharmacy and Pharmacology 2009;61: 42.
Dr Sangeeta Tanna
T: +44 (0)116 2078274
Dr Graham Lawson
T: +44 (0) 116 2577129