Dr Avninder S Bhambra

Job: Programme Leader for Biomedical Science BSc (Hons) and Senior Lecturer

Faculty: Health and Life Sciences

School/department: School of Allied Health Sciences

Address: De Montfort University, The Gateway, Leicester, LE1 9BH

T: 0116 2078792

E: abhambra@dmu.ac.uk

W: www.dmu.ac.uk/alliedhealthsciences

 

Publications and outputs 


  • Can new digital technologies support student retention and engagement?
    Can new digital technologies support student retention and engagement? Pena-Fernandez, A.; Randles, Michael J.; Young, Christopher N. J.; Potiwat, N.; Bhambra, Avninder S. University students in their first year face a myriad of challenges such as information overload, poor individual attention and/or minimal interaction with their peers, which can impede their progress through higher education. These challenges, together with other factors, have an impact on student retention and progression that needs to be addressed. We have established a range of strategies to improve retention and progression of new Biomedical Science (BMS) students at De Montfort University (DMU, UK) in 2016/17, including an intensive induction week with social and networking events with academics to enhance the development of constructive relationships. We have also increased the number of lectures on foundation in biology, chemistry and maths, introduced more tutorials and created “surgery” hours or weekly drop-in sessions in each module. These strategies could have been translated into a reduction in the percentage of students that abandon their BMS studies after their first year from 10.3% (24 students) in 2015/16 to 6.5% (13 students) in 2016/17, according to DMU reporting software (Tableau). However, we have noted that some of our BMS students require more basic support in STEM subjects (biology and chemistry), particularly those students that enter from the Business and Technology Education Council (BTEC) pathway, despite having met university-set entry requirements and the modifications to the curriculum to cater for such students. To address these limitations, we have started to develop a complete e-learning package designed to enhance learning and underpin the fundamental concepts of biology and biochemistry. The development of the DMU e-Biology package started in 2017 and covers the specifications for AS and A level described by the Assessment and Qualifications Alliance (AQA, 2017) for human biology as well as the basic concepts delivered in our first year modules. The DMU e-Biology also has interactive case studies related to topics of interest for our students, such as alcohol abuse and biomarkers of disease in clinical samples, to encourage self-learning and autonomous work on the part of the user. The main aim of the virtual case studies is to facilitate the development of students’ abilities to critically evaluate and use evidence from the literature, skills that are invaluable to any scientist and indeed key for future generations of biomedical scientists. The final package will be publicly available on the DMU website (http://parasitology.dmu.ac.uk/ebiology/home.htm) in 2018, after reviewing student feedback. The availability of this resource prior to students starting their course may enable earlier engagement and improve student retention.
  • Specialized Plant Metabolism Characteristics and Impact on Target Molecule Biotechnological Production.
    Specialized Plant Metabolism Characteristics and Impact on Target Molecule Biotechnological Production. Matsuura, H.N.; Malik, S.; de Costa, F.; Yousefzadi, M.; Mirjalili, M. H.; Arroo, R. R. J.; Bhambra, Avninder S.; Strnad, M.; Bonfill, M.; Fett-Neto, A.G. Plant secondary metabolism evolved in the context of highly organized and differentiated cells and tissues, featuring massive chemical complexity operating under tight environmental, developmental and genetic control. Biotechnological demand for natural products has been continuously increasing because of their significant value and new applications, mainly as pharmaceuticals. Aseptic production systems of plant secondary metabolites have improved considerably, constituting an attractive tool for increased, stable and large-scale supply of valuable molecules. Surprisingly, to date, only a few examples including taxol, shikonin, berberine and artemisinin have emerged as success cases of commercial production using this strategy. The present review focuses on the main characteristics of plant specialized metabolism and their implications for current strategies used to produce secondary compounds in axenic cultivation systems. The search for consonance between plant secondary metabolism unique features and various in vitro culture systems, including cell, tissue, organ, and engineered cultures, as well as heterologous expression in microbial platforms, is discussed. Data to date strongly suggest that attaining full potential of these biotechnology production strategies requires being able to take advantage of plant specialized metabolism singularities for improved target molecule yields and for bypassing inherent difficulties in its rational manipulation. The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link
  • The synthesis of chalcones as anticancer prodrugs and their bioactivation in CYP1 expressing breast cancer cells
    The synthesis of chalcones as anticancer prodrugs and their bioactivation in CYP1 expressing breast cancer cells Ruparelia, K. C.; Ljaza, T.; Ankrett, D. N.; Wilsher, Nicola Elizabeth; Lodhia, S.; Beresford, Kenneth J. M.; Bhambra, Avninder S.; Arroo, R. R. J.; Potter, G. A.; Butler, P. C.; Tan, Hoon Leong; Zeka, K. Abstract: Background: Although the expression levels of many P450s differ between tumour and corresponding normal tissue, CYP1B1 is one of the few CYP subfamilies which is significantly and consistently overexpressed in tumours. CYP1B1 has been shown to be active within tumours and is capable of metabolising a structurally diverse range of anticancer drugs. Because of this, and its role in the activation of procarcinogens, CYP1B1 is seen as an important target for anticancer drug development. Objectives: To synthesise a series of chalcone derivatives based on the chemopreventative agent DMU-135 and investigate their antiproliferative activities in human breast cancer cell lines which express CYP1B1 and CYP1A1. Method: A series of chalcones were synthesised in yields of 43-94% using the Claisen-Schmidt condensation reaction. These were screened using a MTT assay against a panel of breast cancer cell lines which have been characterised for CYP1 expression. Results: A number of derivatives showed promising antiproliferative activities in human breast cancer cell lines which express CYP1B1 and CYP1A1, while showing significantly lower toxicity towards a non-tumour breast cell line with no CYP expression. Experiments using the CYP1 inhibitors acacetin and 􀀁-naphthoflavone provided supporting evidence for the involvement of CYP1 enzymes in the bioactivation of these compounds. Conclusions: Chalcones show promise as anticancer agents with evidence suggesting that CYP1 activation of these compounds may be involved. The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link
  • Building a DMU e-Biology resource for health sciences’ students.
    Building a DMU e-Biology resource for health sciences’ students. Pena-Fernandez, A.; Sgamma, Tiziana; Young, Christopher N. J.; Randles, Michael J.; del Aguila, C.; Hurtado, C.; Evans, M. D.; Potiwat, N.; Izquierdo, F.; Pena, M. A.; Coope, J.; Armstrong, M.; Bhambra, Avninder S. The BSc Biomedical Science (BMS) programme at De Montfort University (DMU, Leicester, UK) is accredited by the Institute of Biomedical Science (IBMS). Students enrolled within this programme acquire highly sought after skills related with human health sciences to work in: pathology departments in hospitals; research institutions; biotechnology and pharmaceutical industries; and the education sector to name a few. The degree recruits a large number of students with currently around 600 students enrolled on this programme at DMU. Despite pre-entry requirements of knowledge of subjects related to human biology, biology or chemistry, we have noted that first year students require basic support in STEM subjects (biology, chemistry and mathematics) in modules such as “Basic Microbiology”, “Basic Anatomy and Physiology” and “Chemistry for the Biosciences”. This support is especially necessary for students that come from non-traditional routes such as Business and Technology Education Council (BTEC) routes. Moreover, usually topics related with microbiology and human diseases are challenging for students, often causing stress impacting their overall performance and experience. A group of BMS academics at DMU in conjunction with universities in the European Union (EU; e.g. University of San Pablo CEU, Spain) have started to design, create and develop a series of e-learning resources or units in human biology and BMS for undergraduate students that study health sciences degrees in the EU. These units are being uploaded onto the DMU web server (http://parasitology.dmu.ac.uk/) and will be only accessible for students from participating universities during the first phase of this project (2017/18 course) in which comprehensive feedback will be collected. This web server space has three sections or modules (theoretical section, virtual laboratory and microscope) in which the new e-learning resources will be preliminary accommodated. These units will be interactive and easy to follow, and will cover basic human biology (e.g. cells, cell structure), human anatomy and physiology, histology and basic microbiology, which will be embedded in a theoretical module named DMU e-Biology within the above URL link. They will include formative assessments and case studies throughout each unit. In addition, a series of practical units are being developed which describe routine practical elements in any biomedical laboratory such as laboratory materials, pipetting, molecular techniques (e.g. PCR), cell culture (e.g. use of biological safety cabinet) and histological techniques (e.g. use of microtome, staining techniques). The development of this teaching and learning resource will cover a gap in the traditional teaching and learning methods that are currently used and provided in the participating universities. The DMU e-Biology will aid to our undergraduate students to gain knowledge in human biology and microbiology by promoting self-learning. We consider that the DMU e-Biology will help overcome spatiotemporal, equipment and resource barriers. Additionally, it may help student retention as currently about a 10% of our first year students fail to continue BMS at DMU. Finally, the creation of the DMU e-Biology will also provide support to the DMU Student Retention and Attainment Strategy 2016-2020 through the DMU Student Learning Hub, which is currently under development. The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link
  • A meta-analysis study of antimicrobial resistant E. coli in the environment.
    A meta-analysis study of antimicrobial resistant E. coli in the environment. Knight, S.; Bhambra, Avninder S.; Lobo-Bedmar, M. C.; Pena-Fernandez, A. There is increasing evidence that urban animals could play a significant role in the spread of antibiotic-resistant (AR) bacterial infections. Wild birds and pigeons have been found as carriers of multidrug-resistance Escherichia coli (E. coli) in urban ecosystems, which could threaten public health. Although AR bacteria pose challenges to healthcare systems, little is known about the prevalence and distribution of such bacteria in the environment, particularly in the built-up environment. The aim of this study was to review the literature to identify what is known so far and to identify possible animal species that should be targeted in urban environments as part of the national and international response to tackle the AR phenomenon. A systematic review was performed following the Cochrane guidelines to identify peer-reviewed articles investigating AR strains of E. coli published from January 2006 onwards. Eligible studies were selected based on inclusion criteria: carried out in urban areas in Europe; determined E. coli in isolates from urban animals by molecular methods; and results were clear and easy to extract to determine the pooled prevalence according to previous methodologies. Only 18 studies were identified as eligible and were subjected to the meta-analysis following the Cochrane recommendations. The results have highlighted that the occurrence of AR E. coli in Europe has significantly increased since 2014. The study has indicated a different occurrence of such bacteria in urban environments in Europe. Thus, higher prevalence was observed in the Netherlands, Portugal and Spain, meanwhile Latvia and Sweden had the lowest. Finally, urban avian (pigeons and gulls) and canine (domestic) species have been identified as the most likely carriers of AR E. coli in urban environments. Although these results should be considered as preliminary, special attention should be drawn to consider urban animal species in any intervention to reduce the AMR phenomenon.
  • Pyrogallol is the main antibacterial compound in the aqueous extract of Boswellia dalzielii bark
    Pyrogallol is the main antibacterial compound in the aqueous extract of Boswellia dalzielii bark Alfa, Hajara; Arroo, R. R. J.; Walsh, Susannah E.; Ruparelia, K. C.; Bhambra, Avninder S.; Slater, A. Plants belonging to the genus Boswellia (Burseraceae) have long been appreciated for their pharmacological properties such as anti-inflammatory, antioxidative and anticancer activities1. In the Northern part of Nigeria, the bark of B. dalzielii Hutch. is an important ingredient for the treatment of infections2. To identify water-soluble antibacterial compounds, powdered bark was macerated in water for 6 to 24h at a range of temperatures. The aqueous extracts were subsequently fractionated by column chromatography, and the fractions were initially screened against wild type and methicillin resistant strains of Staphylococcus aureus. Then, minimum inhibitory concentrations of purified fractions were determined using series of concentrations from 2 mg/mL to 8 μg/mL. Results showed that longer maceration resulted in stronger antibacterial activity. With the aid of NMR and accurate mass analysis, pyrogallol was identified as the main antibacterial agent, with MIC values ranging from 24-28 μg/mL for MRSA to 34-36 μg/mL for wild-type S. aureus. Gallic acid was found to play a lesser role (MIC >200 μg/mL). Pyrogallol was found not to be a plant secondary metabolite, but a metabolic product from microbial degradation of gallic acid from the bark. Only two bacterial species could be isolated from the plant material, which were identified as Raoultella planticola and Enterobacter cloacae. Of these two micro-organisms, R. planticola was shown to be responsible for the production of pyrogallol. This is an example of bacterial allelopathy, which results in an increased efficacy of the aqueous extract.
  • Building on-line materials for teaching parasitology to health sciences’ students: initial impressions.
    Building on-line materials for teaching parasitology to health sciences’ students: initial impressions. Pena-Fernandez, A.; Ollero, M.; Fenoy, S.; Magnet, A.; Mackenzie, S.; Pena, M. A.; Izquierdo, F.; Hurtado, C.; Ioannou, M.; Bornay, F.; Halliwell, R.; Acosta, L.; Torrus, D.; Singh, Harprit; Sgamma, Tiziana; Evans, M.; Bhambra, Avninder S.; Baho, S.; del Aguila, C. Background: It is widely recognised that the use of web-based teaching resources is an increasingly important method for delivering education, and it will be particularly important in the near future due to the progressively increasing number of health science students and the current number of academics in the “European Higher Education Area”. The study of parasitology and infectious diseases is essential to build professionals in the health sector with the key knowledge and skills to face global public health threats such as food-, water- or vector-borne infectious diseases outbreaks. However, the current time dedicated to the teaching of this discipline in all health sciences degrees at De Montfort University (DMU, Leicester, UK) is very little or non-existent depending on the degree/master. Methods: An innovative teaching group at DMU is trying to fill this gap in the currently available teaching offer in line with new trends in global health education, the large number of students enrolled in any health degree and the increasing number of students that would like to study this discipline (but due to different commitments do not have enough time or resources to study on a full time basis). Thus, an innovative teaching group from different EU Universities (DMU and the Spanish universities: University of San Pablo CEU, University of Alcalá, and University Miguel Hernández de Elche) and clinicians (University Hospitals of Leicester, UK) have started to design, create and develop a complete on-line package in Parasitology for undergraduate and postgraduate students that study health sciences. Results: The e-Parasitology package will be accessible through the DMU website (http://parasitology.dmu.ac.uk) in 2017 and will be focused on infection, prevention and treatment of major and emerging parasitological diseases. Conclusions: This teaching resource will aid our undergraduate and postgraduate students to gain a significant knowledge in parasitology by promoting self-learning and internationalization. This poster will explore one of the first mini-modules developed so far related with Toxocara, a helminthiasis with prevalence rates that can reach as high as 40% or more in parts of the world, and the challenges for its development.
  • Health Effects and Risks of Caffeine, Theobromine and Theophylline
    Health Effects and Risks of Caffeine, Theobromine and Theophylline Pena-Fernandez, A.; Evans, M. D.; Bhambra, Avninder S. Methylxanthines, namely caffeine, theobromine and theophylline are found in several beverages and food products such as coffee, cocoa, tea and cola drinks. These substances can provide benefits to humans but also prove harmful mainly when consumed in high amounts. However, the beneficial and deleterious effects of methylxanthines are controversial in research findings, largely because of inconsistencies within research conducted and factors associated within studies. For instance, some authors have reported methylxanthines could have potential antioxidant activity under physiological conditions but opposing studies do not support these claims. Factors that influence the effects of methylxanthines include age, gender and health status of the individual. In addition to these, other factors such as doses, consumption of drugs or pharmaceuticals, alcohol and tobacco habits and diet also play a role in methylxanthine effects and metabolism. Therefore, our review is to provide a clearer understanding of the beneficial/adverse effects of methylxanthines in humans. A description of the physiological and toxicological effects of these methylxanthines is provided.
  • Synthesis and antitrypanosomal activities of novel pyridylchalcones
    Synthesis and antitrypanosomal activities of novel pyridylchalcones Bhambra, Avninder S.; Ruparelia, K. C.; Tan, Hoon Leong; Tasdemir, D.; Burrell-Saward, H.; Yardley, Vanessa; Beresford, Kenneth J. M.; Arroo, R. R. J. A library of novel pyridylchalcones were synthesised and screened against Trypanosoma brucei rhodesiense. Eight were shown to have good activity with the most potent 8 having an IC50 value of 0.29 M. Cytotoxicity testing with human KB cells showed a good selectivity profile for this compound with a selectivity index of 47. Little activity was seen when the library was tested against Leishmania donovani. In conclusion, pyridylchalcones are promising leads in the development of novel compounds for the treatment of human African trypanosomiasis (HAT). Collaboration with the London School of Hygiene and Tropical Medicine. The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.
  • Novel Fluorinated Benzimidazole-Based Scaffolds and their Anticancer Activity in vitro
    Novel Fluorinated Benzimidazole-Based Scaffolds and their Anticancer Activity in vitro Bhambra, Avninder S.; Edgar, Mark; Elsegood, Mark R. J.; Horsburgh, Lynne; Kryštof, Vladimir; Lucas, Paul D.; Mojally, Mariam; Teat, Simon J.; Warwick, Thomas G.; Weaver, George W.; Zeinali, Fatemeh A small library of twelve, structurally diverse, fluoroaryl benzimidazoles was prepared using a simple synthetic strategy employing SNAr reactions. This allowed rapid assembly of heterocyclic structures containing linked and tethered fluoroaryl benzimidazoles. X-ray crystal structures of seven compounds were obtained including those of two macrocyclic compounds containing 21- and 24-membered rings. Three tethered fluoroaryl benzimidazole derivatives demonstrated micromolar inhibition against K-562 and MCF-7 cell lines. These compounds, in addition to 1-tetrafluoropyrid-4-yl-2-tetrafluoropyrid-4-ylsulfanyl-1H-benzimidazole, also demonstrated micromolar inhibition against G361 and HOS cell lines. Two of the compounds were found to activate caspases leading to apoptosis. The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.

Click here for a full listing of Dr Avninder Bhambra's publications and outputs

Key research outputs

  • Arroo RRJ, et al. (2014) Phytoestrogens as natural prodrugs in cancer prevention: towards a mechanistic model. Phytochemistry Review.
  • F. Marken, A. S. Bhambra, D. Kim, R. J. Mortimer, S. J. Stott (2004). Electrochemical reactivity of TiO2 nanoparticles adsorbed onto boron-doped diamond surfaces. Electrochemistry Communications 6, 1153-1158.

Research interests/expertise

  • Development and biological evaluation of anticancer therapies
  • Development and biological evaluation of antimicrobial therapies for neglected tropical diseases and drug-resistant bacteria  
  • Medicinal Chemistry 
  • Biorganic Chemistry
  • Natural Product Synthesis
  • Heterocyclic Chemistry
  • Phytotherapy
  • Analytical Chemistry

Areas of teaching

  • Anticancer Therapies
  • Antimicrobial Therapies
  • Molecular Sciences
  • Biochemistry
  • Research Projects
  • Professional Skills

Qualifications

  • BSc (Hons)
  • PhD
  • PGCert
  • MRSC

Courses taught

  • Biomedical Science
  • Medical Science
  • Advanced Biomedical Science
  • Pharmacy 

Membership of professional associations and societies

  • Royal Society of Chemistry
  • Phytochemical Society of Europe
  • Editorial Board member for Cogent Chemistry
  • Fellow of the Higher Education Academy

Current research students

  • Supervising 3 PhD students 

Please contact for MSc or PhD opportunities.

Externally funded research grants information

  • Royal Society of Chemistry - Researcher Mobility Fellowship (2014) in collaboration with the University of Barcelona developing anticancer therapeutics.

Internally funded research project information

  • HEIF – Synthesis of Novel Antiprotozoal Agents for the Treatment of Neglected Tropical Diseases (2013).

Published patents

  • Novel Trypanocidal Compounds. Patent Application Number 1400366.9.

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