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Dr Shivanthi Samarasinghe

Job: Senior Lecturer

Faculty: Health and Life Sciences

School/department: School of Allied Health Sciences

Research group(s): Biomedical and Environmental Health

Address: De Montfort University, The Gateway, Leicester, LE1 9BH.

T: +44 (0)116 207 8870

E: ssamarasinghe@dmu.ac.uk

W: www.dmu.ac.uk/alliedhealthsciences

 

Personal profile

Dr Shivanthi Samarsinghe is a senior lecturer in microbiology and molecular genetics/genomics in the School of Allied health Sciences, De Montfort University. She obtained her BSc (Hons) in Chemistry/Biochemistry, and completed her PhD, in Microbial Molecular Genetics in 2008.

Dr. Samarasinghe’s doctoral work focused studying on the regulation of gene expression in Escherichia coli and her postdoctoral work (2011) expanded her interest into Eukaryotic regulation of gene expression in Saccharomyces pombe.

 Research Interest: Molecular biology of antibiotic resistant microbial pathogens;

The Molecular Microbiology Research Theme focuses on understanding the molecular basis of the antibiotic resistant microbial pathogens. This involves characterisation of the virulence genes at the molecular level, how they acquire resistance genes (pathogen’s horizontal gene transfer) and analyse the mechanism by which these pathogenic microbes confer antimicrobial resistance. Further, the research theme involves developing the novel molecular methods; PCR based diagnostic methods for diagnosing the antibiotic resistant microbial pathogens at the early stage of their infectious diseases

Inclusion of REF: Selected for inclusion of REF 2014, two outputs of  research within the science based discipline achieved four star, and one output in the same discipline achieved three star.

Research group affiliations

  • Biomedical and Environmental Health
  • Bimolecular Technology
  • Infectious Disease Research Group

Publications and outputs 

Click here for a full listing of Shivanthi Samarasinghe's publications and outputs|.

Key research outputs

  • Samarasinghe, S. et al, (2008)
  • Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR, Nucleic Acids Research, 1–10, doi:10.1093/nar/gkn119
  • Funaya, C. and Samarasinghe, S. et al, (2012)
  • Transient structure associated with the spindle pole body directs meiotic microtubule reorganization in S.pombe, Current Biology, 22, 1-10, D-11-01295R
  • Elrobh, M., Webster, C., Samarasinghe, S. et al (2012)
  • Two DNA sites for MelR in the same orientation are sufficient for optimal MelR-dependent repression at the Escherichia coli melR promoter, FEMSLE-12-07-0641.R1

Research interests/expertise

  • Key Research theme: Molecular biology of antibiotic resistant microbial pathogens;
  • The Molecular Microbiology Research Theme focuses on understanding the molecular basis of the antibiotic resistant microbial pathogens. This involves characterisation of the virulence genes at the molecular level, how they acquire resistance genes (pathogen’s horizontal gene transfer) and analyse the mechanism by which these pathogenic microbes confer antimicrobial resistance. Further, the research theme involves developing the novel molecular methods; PCR based diagnostic methods for diagnosing the antibiotic resistant microbial pathogens at the early stage of their infectious diseases
  • Application of molecular tools for diagnosis of microbial infectious diseases
  • Understanding the molecular basis of regulatory pathways in microbial pathogens

 

Areas of teaching

  • Basic Microbiology for Biomedical Science
  • Medical Microbiology
  • Molecular Genetics and Genomics

Qualifications

  • Postdoctoral Research Associate in Molecular Cell Biology: Department of Biochemistry, University of Leicester (2008 -2011)
  • PhD in Microbial Molecular Genetics: School of Biosciences, University of Birmingham, 2008
  • BSc.Chemistry and Biochemistry: Institute of Chemistry, Sri Lanka, 2001

Honours and awards

  • The Vice-Chancellor’s Future Research Leader Award- (DMU, 01/2014).
  • Postdoctoral Research Associate in Molecular Cell Biology;Dept. of Biochemistry, University of Leicester, BBSRC funded postdoctoral research (01/08/2008- 31/07/2011).
  • PhD studentship in      Microbial Molecular Genetics awarded by The School of Biosciences,      University of Birmingham and the Darwin Trust of Edinburgh      07/2004-07/2008.
  • Awarded for best scientific paper submitted, Science Research Conference, Association of Professional Sri Lankans (APSL), London, 11/2011

Membership of professional associations and societies

  • Institute of Biomedical Science: Fellowship (FIBMS); (no: 402725)  01/2015-01/2016
  • Society for Applied Microbiology: Full Ordinary member (no: 503356) 01/2013-01/2016.
  • Healthcare Infection Society: Full Ordinary member (no: 9030) 07/2014- 07/2016.

 

Professional licences and certificates

Fellow of Institute of Biomedical Sciences (2015)- FIBMS

 

Projects

  • Bioinformatics analysis of the transcription regulators in multi-drug resistant microbial pathogens.
  • Analysis of Quorum Sensing Regulations in the multi-drug resistant Europathogenic E. coli.
  • Development of Quorum –based anti-virulence therapeutics targeting Gram-negative bacteria.
  • Molecular diagnostics of antibiotic resistant microbial pathogens.

Conference attendance

Conferences attended, submitted abstracts, posters and oral presentations:

  • Genotypic analysis on the multidrug resistant uropathogenic E. coli.  BSAC Antimicrobial resistance Mechanisms Conference, Birmingham-11/2014(poster presentation).

  •  Analysis of unique and specific genetic markers for diagnosis of antibiotic resistant, pathogenic E. coli encoding resistance to the third generation of antibiotic chefotaxime (oral and poster presentation). European Society for translational Medicine.  Annual International European congress in Translational Medicine, Vienna, Austria, 22-25 September, 2014 ( Funded by DMU Future Research Leaders scheme 2014)

  • RNA Polymerase Workshop, York, March 2005
  • RNA Polymerase Workshop, Birmingham, March 2006
  • Journal of Biochemical society 100th Century research conference, Glasgow, June, 2006
  • RNA Polymerase Workshop, London, March 2007
  • Cyclic Nucleotide Signaling Meeting, Durham, April 2007
  • FASEB, Transcription regulation in prokaryotes, Vermont, U.S.A., June, 2007
  • Gene expression and analysis, Biochemical Society, Manchester, March, 2008
  • S. pombe Club, MRC, London, March, 2009
  • British meiosis meeting, University of Sussex, March, 2009
  • S. pombe meeting, University of Tokyo, Japan, 2009
  • British Yeast group meeting, University of Oxford, March, 2010
  • British meiosis meeting, University of Leicester, March, 2010
  • British Society for Cell Biology Autumn meeting , September, 2010
  • British meiosis meeting, University of Sheffield, April, 2011
  • Science Research Conference, Association of Professional Sri Lankans (APSL), London, November, 2011

Recent research outputs

Shivanthi Samarasinghe (2015). Analysis of unique and specific genetic markers for diagnosis of antibiotic resistant, pathogenic E. coli encoding resistance to the third generation of antibiotic chefotaxime. New Horizons in Translational Medicine, 2, page 70; DOI: http://dx.doi.org/10.1016/j.nhtm.2014.11.051|

 

Consultancy work

  •  Ms. Andrea O’ Corner, Senior Biomedical Scientist, Clinical microbiology unit,      Northampton General Hospital, NHS trusts Northampton (2014).

PCR based molecular diagnostic method and double disk synergy method on analysing the multidrug resistant uropathogenic E. coli.

  • Dr David Jenkins, (Consultant      Medical Microbiologist, Lead Infection Control Doctor, and Deputy Director      of Infection Prevention and Control), and Dr Chris Holmes, (Senior      Clinical Scientist ) Pathology, University Hospitals of Leicester.

PCR based molecular diagnostic (RT-PCR) on analysing the multidrug resistant, (carbapinamases resistant) uropathogenic bacteria (2014-todate).

 

Current research students

Molecular Characterisation and prevalence analysis of CTX-M antibiotic resistant E. coli in Leicestershire. DMU PhD studentship; first supervisor (10/2014-04/2018), partnership withClinical Microbiology, University Hospitals of Leicester NHS Trust.

Molecular Characterisation of the Quorum sensing genes in the multi-drug resistant uropathogenic E. coli.Ministry of Higher Education, Iraq, funded PhD studentship; first supervisor(04/2015-04/2019).

3.     Analysis of virulence gene expression via quantitative Real-time PCR during biofilm formation in MRSA; DMUPhD studentship, second supervisor collaborative research project with Dr. Katie Laird, (10/2014-01/2016).

4.     Molecular detection and rapid diagnosis of the antibiotic resistant E. coli pathogens in the companion animals (cats and dogs). DMUPhD studentship, second supervisor (10/2013-01/2016).

5.     The effect of novel synthetic antimicrobials on virulence gene expression of the antibiotic resistant Gram-negatives. DMUPhD studentship, second supervisor (10/2015-10/2019).

Externally funded research grants information

  • Industrial Project grant (Shawabe technologies) Analysis antimicrobial effect of natural oil extracted from plant based product on Gut Microflora (co- Investigator, collaboration with Dr. Katie Laird and Dr Benjamin Gronier, HLS, DMU‎ (11/2014-01/2016).

 

  • HCED Iraq funded PhD studentship:Analysis of the Quorum sensing (QS) gene expression  and eveluate the relationship between QS and virulance potential / antibiotic resistance in the pathogenic Gram negative organism, Pseudomonas aeruginosa. Principal Investigator/First supervisor (01/04/2015-01/04/2018).

 

Internally funded research project information

 

  • Small project Scheme to establish the molecular microbiology research lab (£5000) Faculty of Health and Life Sciences, 05/2013, PI).
  • Research Investment Capital Funding for Real-Time PCR (£12,600, Faculty of Health and Life Sciences, 10/2013, PI).
  • DMU fees only PhD      studentship; Molecular Characterisation and development of molecular      diagnostic markers for CTX-M antibiotic resistant E. coli. first      supervisor/PI (10/2014-10/2017)
  • DMU fees only PhD studentship; Analysis of virulence gene expression via quantitative Real-time PCR during biofilm formation in MRSA. Co-investigator/ second supervisor. (2014-2016).

 

Case studies

In my postdoctoral work (Department of Biochemistry, University of Leicester, under the Principal Investigator, Dr. Kayoko Tanaka) I have contributed to the following research project.

Scientists make stunning inner space observations
Web site >|

Posted by pt 91 at May 01, 2012 10:19 AM | Permalink

High-powered microscopes reveal workings of the cell – results could impact treatment of Down’s Syndrome, lissencephaly (a brain formation disorder) or cancer.

Shavanthi-Samarasingheshivanthi-samarsinghe-antibiotic-guardian-logo

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